Research Paper Volume 13, Issue 4 pp 5034—5054

Figure 2. GATA2 transcriptionally repressed TRIM29 expression. (A, B) The low frequencies of mutation (A) (1%) and amplification (B) (<1%) for TRIM29 in the TCGA database. (C, D) Analysis of possible physical associations of GATA2 with regions in the TRIM29 promoter. ChIP assays were performed with GATA2 antibody. IgG served as the negative control. (E) Relative luciferase activity in 293T cells transfected with reporter constructs containing the TRIM29 promoter. Luciferase reporter activity was normalized to Renilla luciferase activity. (F) The mRNA expression level of TRIM29 in cells transfected with GATA2 plasmid was detected using qPCR. (G) GATA2 was negatively correlated with TRIM29. (H) Representative images of GATA2 expression in right-sided colorectal cancer (RSCC) and left-sided colorectal cancer (LSCC). RSCC had a higher frequency of loss of GATA2 than did LSCC. The error bars represent the SEM. *P < 0.05, reporter activity was normalized to Renilla luciferase activity. ChIP assay and luciferase reporter assay (D–F) repeated at least 3 times.