Figure 5. Regulation of functional proteins by TiO2 NPs in corneal endothelial cells. Immunofluorescence analysis of the expression of ZO-1 (A), Na-K-ATPase (B) and β-catenin (C) in primary corneal endothelial cells with TiO2 NPs treatment, H2O2 treatment or Nrf2 overexpression. bar = 20 μm. *P<0.05, **P<0.01, ***P<0.001.