Figure 4. Lupeol reduces oxidative stress in zebrafish and in mice with ALI. (A) Fluorescence micrographs of ONOO- generation in the control zebrafish larvae and the zebrafish larvae treated with LPS or lupeol. N=3-5, figures are magnified as 400x, bar=200 μm. (B) Quantification of the amounts and distribution of ONOO-. Data are exhibited as mean ± SEM. (C, D) NT immunohistochemical staining of mice livers and its quantitative analysis. (E, F) Western blots and quantitative results for iNOS. (G, H) Immunofluorescence staining and quantification for iNOS. All data are shown as the mean ± SEM, n=3-4 group, *P<0.05 vs control group, #P<0.05 vs model group. Figures are magnified as 200x, bar=50μm.