Research Paper Volume 12, Issue 24 pp 24836—24852

Figure 3. miR-106b exerts an anti-angiogenic effect and impairs retinal endothelial cell migration. (A) Western blot of VEGFA and β-actin from control choroids and 3 days after burns, and (B) quantification (n=4). (C) Western blot of HIF1α and β-actin from control choroids and 3 days after burns, and (D) quantification (n=4). (E) Schematic of ECIS cell migration assay procedure. HRMEC ECIS with (F) LV.miR-106b (n=4), (G) LV.shVEGFA (n=4) and (H) LV.shPERK (n=4) compared to LV.shGFP control. (I) HRMEC scratch assay infected 72h with LV.shGFP, LV.miR-106b, LV.shVEGFA and LV.shPERK at T0h and after 8h. (J) Migration area quantification of scratch assay with LV.miR-106b (n=4), LV.shVEGFA (n=4), and LV.shPERK (n=4) compared to LV.shGFP. (K) Sprouting assay with choroid explants infected with LV.shGFP, LV.miR-106b, LV.shVEGFA, and LV.shPERK. (L) Sprouting area quantification with LV.miR-106b (n=9), LV.shVEGFA (n=9), LV.shPERK (n=8) compared to LV.shGFP control. Scale bar = 500 μm. Data are expressed as mean ± S.E.M. Unpaired Two-tailed Student’s t-test was used for the analysis of groups of 2, and one-way ANOVA with Bonferroni post-hoc test was performed on groups of 3 or more, *P <0.05; **P<0.001; ***P<0.0001.