Research Paper Volume 13, Issue 1 pp 1132—1152

Figure 9. The therapeutic effect of IL-6 and VEGF is partially mediated via HO-1 dependent mechanism. (A) The protein levels of HO-1 in IEC-6 were detected by western blot assays on day 3 after radiation with β-tubulin as the internal control. Data represent the mean ± SD (n=3). ^*, P < 0.05 versus IR + DMEM-F12. ^#, P < 0.05 versus Control. ^##, P < 0.05 versus IR + IL-6 + VEGF. (B) Inhibition of HO-1 partially inhibits the therapeutic effect of IL-6 + VEGF on apoptosis of IEC-6. The ratio of apoptosis IEC-6 cells was determined by PI/Annexin V staining on day 3 after radiation. Data represent mean ± SD of three independent experiments. ^**, P < 0.05 versus IR + IL-6 + VEGF. ^##, P < 0.05 versus IR + DMEM-F12. (C) The ratio of apoptosis IEC-6 cells was determined by PI/Annexin V staining on 1, 3, 5, 7 days of the experiment. Data represent mean ± SD of three independent experiments. ^##, P < 0.05 versus IR+ IL-6 + VEGF. (D) The activity of NF-κB p65 in IEC-6 was measured by an ELISA-based assay directed against the p65 subunit of NF-κB. Data represent the mean ± SD (n=3). ^##, P < 0.05 versus IR + DMEM-F12. ^**, P < 0.05 versus IR + IL-6 + VEGF. (E) Cell viability was detected by CCK-8 from day 0 to day 7 after radiation. ^##, P < 0.05 versus IR + IL-6 + VEGF + ZNPP. Data represent mean ± SD of three independent experiments. All data were analyzed by t-test or one-way ANOVA except as otherwise indicated.