Research Paper Volume 13, Issue 1 pp 1096—1119

Figure 8. The eNOS/cGMP/PKG pathway activated PFs by inhibiting FBXW7-induced mTOR ubiquitination. (A–C) Six days after transfection, (A) the ovarian morphology, (B) the total number of oocytes and (C) the numbers of PFs and GFs/the total number of follicles were analyzed (n=8). Scale bar, 40 μm. (D) Immunofluorescence staining of FOXO3a in the empty vector and OE vector groups. Scale bar, 40 μm. (E) The number of oocytes with CL-FOXO3a/the total number of oocytes was analyzed in each group (n=8). (F) The number of PFs with CL-FOXO3a/the total number of PFs was analyzed in each group (n=8). (G) The negative correlation between the relative mRNA levels of FBXW7 and PKG. (H) The negative correlation between the relative mRNA levels of FBXW7 and the levels of cGMP. (I) The relative mRNA levels of FBXW7 in ovaries treated with the vehicle, SN, NM, 8Br or KT. *, ** and *** denote statistical significance at p < 0.05, p < 0.01 and p < 0.001, respectively. (J, K) After treatment with the empty vector, empty vector + SN, OE vector or OE vector + SN for six days, (J) the ovarian morphology, and (K) the numbers of PFs and GFs/the total number of follicles were analyzed (n=8). Scale bar, 40 μm. Different letters with the same color denote statistical significance at p < 0.01 (Red letters represent the proportions of PFs, while black letters represent the proportions of GFs).