Research Paper Volume 13, Issue 1 pp 933—943
IRF-1 contributes to the pathological phenotype of VSMCs during atherogenesis by increasing CCL19 transcription
Figure 5. Effects of IRF-1 on VSMCS though CCL19. (A) Detection of overexpression efficiency of IRF-1 plasmid by qRT-PCR. n=5. Knockdown CCL19 reversed IRF-1 induced mRNA expression of inflammatory cytokine IL1-α (B), IL-1β (C), TNF-α (D), IL-6 (E) and the deposition of ECM collagen1 (F), collagen3 (G) and OPN. (H, I) Western blot showed the collagen1, IL-6 and CCL19 protein expression induced by IRF-1, but reduced by sh-CCL19. n = 4. Migration (J) and proliferation (K) of VSMCS of IRF-1 overexpression, with or without sh-CCL19. n = 6. *P<0.05; **P<0.01.