Coicis semen protects against focal cerebral ischemia-reperfusion injury by inhibiting oxidative stress and promoting angiogenesis via the TGFβ/ALK1/Smad1/5 signaling pathway

Jin Du; Guobing Yin; Yida Hu; Si Shi; Jiazhen Jiang; Xiaoyan Song; Zhetao Zhang; Zeyuan Wei; Chaoliang Tang; Haiyan Lyu

doi:doi:10.18632/aging.202194

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Research Paper Volume 13, Issue 1 pp 877—893

Coicis semen protects against focal cerebral ischemia-reperfusion injury by inhibiting oxidative stress and promoting angiogenesis via the TGFβ/ALK1/Smad1/5 signaling pathway

Figure 8. Coicis semen promoted angiogenesis in HUVECs after OGD/RX through upregulation of the TGF-β1-ALK1-Smad1/5 signaling pathway. (A) Representative western blots showing TGF-β1, ALK1, Smad1/5, p-Smad1/5 and VEGF. Quantitative analysis of TGF-β1 (B), ALK1 (C), p-Smad1/5 (D), and VEGF (E) protein expression levels in HUVECs after OGD/RX. Mean ± SD. n = 8. ^aP < 0.05 vs. sham; ^bP < 0.05 vs. DMSO + OGD/RX; ^cP < 0.05 vs. LY2109761 + OGD/RX. Statistical analyses were performed by one-way ANOVA followed by Tukey’s multiple comparison tests.