Research Paper Volume 13, Issue 1 pp 537—554

Figure 2. Overexpression of SNRPB promotes tumor growth in HCC cells. (A, B) qRT-PCR (A) and western blotting (B) were used to confirm the overexpression of SNRPB in SNRPB-transfected 7701 and 7721 cells. 18S or β-Actin served as the loading control. (C) Cell growth rates of empty vector- or SNRPB-transfected 7701 or 7721 cells. (D, E) Representative images of increased colony formation in soft agar (D) and foci formation in monolayer culture (E) induced by SNRPB overexpression in 7701 or 7721 cells. The number of colonies or foci in the vector-transfected and SNRPB-overexpressing groups are summarized in the right panel. (F) Images and growth curves of the xenograft tumors formed in nude mice injected with SNRPB- and empty vector-transfected 7721 cells (n = 5). (G) The weights of xenograft tumors derived from SNRPB- and empty vector-transfected 7721 cells are summarized. (H) IHC staining was used to confirm the level of SNRPB in xenograft tumors. Scale bars = 100 μm. In all panels, *P < 0.05, **P < 0.01, ***P < 0.001.