Research Paper Volume 13, Issue 1 pp 389—410
DOK3 is involved in microglial cell activation in neuropathic pain by interacting with GPR84
Figure 1. Downregulation of DOK3 reduces the levels of inflammatory cytokines in microglia. Microglia were infected with a DOK3-specific short hairpin RNA (shRNA) to establish stably down-regulated DOK3 expression. Total cell lysates of DOK3-shRNA and Scr-shRNA microglia were used to determine the levels of mRNA for DOK3 (A), TNF-α (B), IL-1β (C), IL-6 (D), iNOS (E), P38 (F), CD68 (G), CD11B (H), Arg1 (I), and CD206 (J) by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). N=3 per group, and data are presented as means ± SEM. *p < 0.05, #p < 0.01 vs. Scr-shRNA.