Research Paper Volume 12, Issue 24 pp 25505—25527
Sesn2 attenuates the damage of endothelial progenitor cells induced by angiotensin II through regulating the Keap1/Nrf2 signal pathway
Figure 2. Overexpression of Sesn2 promoted the survival of EPCs after Ang-II treatment. EPCs were transfected with Lenti-Sesn2 or NC before the treatments with Ang-II. (A) The effects of Lenti-Sesn2 were confirmed by using qRT-PCR. (B) The levels of Sesn2 proteins were upregulated significantly by the applications of Lenti-Sesn2. (C) The levels of Keap1, Nrf2, Bax, Bcl-2, Mit cyt-c, Cyto cyt-c, Cleaved-caspase 3 and GAPDH related proteins in EPCs were detected by using Western blotting. (D) The levels of Keap1 proteins in EPCs were detected by using Immunofluorescence. Scale bars = 2 μm. All experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, versus the control. Mit cyt-c: Mitochondria cytochrome C; Cyto cyt-c: Cytoplasm cytochrome C. Data are represented as mean +/- SEM.