Research Paper Volume 12, Issue 24 pp 25505—25527

Figure 1. Identification of EPCs and Ang-II increased Sesn2 and Nrf2 protein expression in EPCs. Identification and exposures of EPCs to Ang-II for prolonged periods of time. (A) EPCs (passage (P) 1) from human umbilical cord. Scale bars = 5 μm and Scale bars = 40 μm. (B) We used immunofluorescence to detect the measures of CD31, CD34, CD134, and VEGFR2. (C) The treatments with Ang-II caused an increased levels of Sesn2 mRNA in EPCs. (D) The levels of Nrf2 mRNA were unchanged in EPCs exposed to Ang-II. (E) The treatments with Ang-II increased the levels of Sesn2 and Nrf2 proteins in EPCs. (F) We used Hoechst 33258 to detect the apoptosis of EPCs and found that the treatments with Ang-II caused an increase in the apoptosis of EPCs. Scale bars = 5 μm. All experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, versus the control. Data are represented as mean +/- SEM. (G) We used Hoechst 33258 staining to detect the apoptosis of EPCs. Scale bars = 5 μm.