Research Paper Volume 12, Issue 24 pp 25469—25486

Figure 1. Increased TNFSF14 expression in mice after UUO surgery. (A) TNFSF14 expression in kidney tissues at the indicated time points in Tnfsf14^+/+ mice after UUO surgery was assessed by qRT-PCR. GAPDH was used as the internal control. (B) The expression of TNFSF14 in the kidney tissues of Tnfsf14^+/+ mice after UUO for 7 days was detected by immunohistochemistry (upper lane, original magnification ×200; lower lane, original magnification ×400). (C) TNFSF14 levels in serum in Tnfsf14^+/+ mice at the indicated time points after UUO surgery were measured by ELISA. The data were representative of the results of three independent experiments. All values are represented as mean ± SEM. Sham group was used as the UUO control. n = 5 per group. ^*P < 0.01, ^**P < 0.01 and ^***P < 0.001.