Research Paper Volume 12, Issue 24 pp 25452—25468

Figure 8. Schematic illustration of the experimental protocol in vivo and in vitro. (A) All the groups underwent the same surgical operation. (1) SHAM: rats were subjected to open chest surgery only; (2) SEVO: rats received 1.0 MAC sevoflurane for 15 min without occlusion; (3) I/R: rats were subjected to 30 min LAD occlusion, followed by 2 h of reperfusion; (4) SPC: rats were subjected to I/R and receiving 1.0MAC sevoflurane for 15 min at onset of reperfusion. (B) Except the SHAM group, all hearts was subjected to 30 min of global ischemia, followed by 2 h of reperfusion. SPC group received 1.0 MAC sevoflurane for 15 min at onset of reperfusion. In the SPC+DMSO group or SPC+OSMI-1 group, in addition to SPC administration, 50 μM DMSO or OSMI-1 was given and present throughout the experiment.