Figure 4.TILRR, the FREM1 isoform 2 transcript, is clinically relevant in breast cancer. (A) The three FREM1 isoforms. (B) TILRR, the gene encoding isoform 2, shows convincing cancer specificity with strong usage and significantly low hazard ratio. (C) TILRR-specific primer of human DNA. (D, E) TILRR expression levels were analyzed by Q-PCR in paired normal and tumor tissues (*P < 0.05, **P < 0.01, ***P < 0.001). (F, G) TILRR immunoblotting (indicated by the FREM1 or TILRR antibody) in paired normal and tumor tissues. The band intensities are normalized to GAPDH.
