Research Paper Volume 12, Issue 17 pp 17343—17352

Figure 4. Overexpression of HOXC9 reverses miR-495-induced inhibition of NSCLC cell growth and migration in vitro. A549 and H1299 cells were transfected with miR-495 mimics or a HOXC9 overexpression vector either singly or in combination. (A) GEPIA analysis of survival of NSCLC patients with high versus low HOXC9 expressing cases. (B) qRT-PCR assay of miR-495 expression in A549 and H1299 cells. Data are means ± SD. ***P < 0.001 vs. controls. (C, D) Western blots of HOXC9 protein expression in A549 (C) and H1299 (D) cells. (E) Relative protein expression is reported as means ± SD. ***P < 0.001 vs. controls; ^###P < 0.001 vs. mimic. (F, G) CCK8 assay of A549 (F) and H1299 (G) cell proliferation. Data are means ± SD. ***P < 0.001; ^#P < 0.05 vs. mimic.(H, I) Colony formation assays of A549 and H1299 cells proliferation. Data are means ± SD. *P < 0.05, ***P < 0.001 vs. controls;^###P < 0.001 vs. mimic. (J, K) Transwell assays of A549 and H1299 cell migration and invasiveness. Data are means ± SD. *P < 0.05,***P < 0.001 vs. controls; ^###P < 0.001 vs. mimic.