Research Paper Volume 12, Issue 16 pp 16304—16325

Figure 3. HOXD-AS1 promoted stemness maintenance and chemo-resistance in CCA. (A) Spheroid forming abilities of transfected QBC939 and CCLP-1 cells were evaluated by spheroid formation assay. (B) The effect of HOXD-AS1 on stem markers (SOX2, OCT4, Nanog) was detected by western blot. (C) The effect of HOXD-AS1 on the treatment of cisplatin and gemcitabine was measured by CCK-8 assay. (D) Mice were subcutaneously injected with QBC939 cells transfected with HOXD-AS1 plasmid and intraperitoneally injected with PBS, cisplatin or gemcitabine. (E) The stem markers (SOX2, OCT4, Nanog) in xenograft tumors were measured by western blot. ^*P < 0.05, ^**P < 0.01.