Research Paper Volume 12, Issue 16 pp 16224—16237

SirT3 activates AMPK-related mitochondrial biogenesis and ameliorates sepsis-induced myocardial injury

class="figure-viewer-img"

Figure 4. Mitochondrial biogenesis is increased by SirT3 via the AMPK pathway. (AC) RNA was isolated from cardiomyocytes, and then transcription of mitochondrial biogenesis parameters was determined. Lentivirus-loaded SirT3 (SirT3-OE) and AMPK agonist (AICAR) were incubated with cardiomyocyte before LPS treatment. Compound C (CC), an antagonist of AMPK, was used to inhibit the activation of AMPK in SirT3-OE–transfected cardiomyocytes. (DF) Immunofluorescence assay for Nrf2 and PGC1α in the presence of LPS. *P < 0.05.