Research Paper Volume 12, Issue 17 pp 16951—16962
Esculentoside A rescues granulosa cell apoptosis and folliculogenesis in mice with premature ovarian failure
Figure 4. Effect of Esculentoside A (EA) on proliferation and apoptosis of ovarian granulosa cells. (A) The expression of KI-67 of granulosa cells was determined by immunohistochemistry of ovarian tissue. KI-67 was used as positive marker of cell proliferation. (B, C) The expressions of proteins including BCL2, KI-67, BAX, CASPASE 3 and CASPASE9 were detected by western blot. The data were expressed as means ± SD (n =5). ***P < 0.001 vs. Control. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. POF.