Figure 3. Effect of Esculentoside A (EA) on reproductive hormone levels in POF mice. (A) The levels of serum luteinizing hormone (LH), FSH, anti-mullerian hormone (AMH) and estradiol (E2) from normal control, POF (EA-treated and EA-null) were detected by ELISA kits. (B–C) The levels of aromatase and FSHR of proteins and mRNA were evaluated by western blot and RT-qPCR, respectively. The data were expressed as means ± SD (n =5). **P < 0.001, ***P < 0.001 vs. Control. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. POF.