Research Paper Volume 12, Issue 17 pp 16837—16851

Figure 6. The cell cycle was arrested in G2/M phase and apoptosis rate was significantly increased with the knockdown of α-tubulin; A possible signaling map was made for SFN-Cys-induced cell cycle arrest and apoptosis in human GBM cells. (A) Immunofluorescence staining of α-tubulin showed the changes of microtubule morphology after treatment with or without SFN-Cys (30 μM) in U87MG/U373MG cells. Green arrows point to microtubules. (B) The expression of α-tubulin was detected by Western blot in U87MG and U373MG cells treated with or without SFN-Cys for 24 h. (C, D) The microtubules were observed via TEM in U87MG/U373MG cells treated with or without SFN-Cys (30 μM) for 24 h. Red arrows point to microtubules. (E, G) Both U87MG and U373MG cells were treated with α-tubulin siRNA for 72 h, and then the cell cycle and apoptosis rate were detected via flow cytometry. (F) The expression of α-tubulin was detected via Western blot after the intervention of α-tubulin siRNA for 72 h. (H) The possible signaling map for SFN-Cys-induced cell cycle arrest and apoptosis in human GBM cells.