Research Paper Volume 12, Issue 17 pp 16837—16851
Sulforaphane-cysteine downregulates CDK4 /CDK6 and inhibits tubulin polymerization contributing to cell cycle arrest and apoptosis in human glioblastoma cells
Figure 3. SFN-Cys decreased the expressions of p-Rb, CDK4 and CDK6; while increased the expressions of p-ERK1/2 and Cyclin D1, and decreased the co-localization of CDK4, CDK6 to Cyclin D1. (A–E): Western blot showed the expressions of p-Rb, Rb, CDK4, CDK6, p-ERK, and Cyclin D1 in both U87MG and U373MG cells treated with SFN-Cys at the indicated concentrations. (F, G) Immunofluorescence and confocal microscopy were employed to observe the co-localization of CDK4, CDK6 to Cyclin D1 in both U87MG and U373MG cells. Scale bar: 75 μm. Data were shown as means ± SD (n ≥ 3). *, P ≤ 0.01.