Research Paper Volume 12, Issue 17 pp 16820—16836
Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines
Figure 4. NETs stimulate HBEs and A549 to express neutrophilic chemokines. (A and B) CXCL1, CXCL2, and CXCL8 expression in the supernatant was analyzed by Luminex and RT-qPCR, after HBEs (or A549) were treated with NETs co-cultured for 24 h. (C and D) Neutrophil migration was analyzed by transwell after cell culture supernatant was induced for 48 h (200X). NET treated supernatant was added to the lower chamber. #: P<0.05, ##: P<0.01, ###: P<0.001, ####: P<0.0001 vs control group. *: P<0.05, **: P<0.01, ***: P<0.001, ****: P<0.0001 vs NA group. ns = no statistical difference vs NETs group.