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Research Paper Volume 12, Issue 16 pp 16021—16034
Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway
Figure 5. MiR-195-5p combines with VEGFA. (A) The target of miR-195-5p was predicted by Targetscan. (B) The binding of miR-195-5p and VEGFA was verified by dual-luciferase reporter assay. (C) BC cells were transfected with miR-195-5p mimics or inhibitors, and the transfection efficiency of miR-195-5p was determined by qRT-PCR. (D) The mRNA expression of VEGFA in T24 and RT4 cells with miR-195-5p mimics or miR-195-5p inhibitors was examined by qRT-PCR. (E) The protein expression of VEGFA in T24 and RT4 cells were identified by western blot. (F) The VEGFA expression in BC tissues and the relationship of VEGFA and miR-195-5p expression were explored. (G) The VEGFA protein expression in T24 cells with miR-195-5p mimics transfection, or miR-195-5p mimics+ pcDNA3.1 co-transfection was determined by western blot. (H–K) Cell proliferation, apoptosis, migration, and invasion of T24 cells were examined by CCK8, Tunel, scratched wound healing and transwell assay, respectively. Data were expressed as the mean ± SD, n = 3. **P < 0.01, ***P < 0.001.