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Research Paper Volume 12, Issue 16 pp 16021—16034
Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway
Figure 2. DLX6-AS1 promotes proliferation and inhibits apoptosis of BC cells in vitro and in vivo. (A) The expression of DLX6-AS1 was over-expressed by pcDNA3.1-DLX6-AS1 or down-regulated by sh-DLX6-AS1 in T24 and RT4 cells, and the transfection efficiency was identified by qRT-PCR. (B) Cell proliferation of T24 and RT4 cells with pcDNA3.1-DLX6-AS1 or sh-DLX6-AS1 transfection was determined by CCK-8 assay. (C, D) The apoptosis of T24 and RT4 cells with pcDNA3.1-DLX6-AS1 or sh-DLX6-AS1 transfection was determined by TUNEL staining assay. (E, F) A subcutaneous BC tumor model (n=6/group) was established by using T24 and RT4 cells to evaluate the anti-tumor effect of shDLX6-AS1, and tumor volume and weight were calculated. Data were expressed as the mean ± SD. ***P < 0.001.