Research Paper Volume 12, Issue 12 pp 11754—11767
miR-107 inhibition upregulates CAB39 and activates AMPK-Nrf2 signaling to protect osteoblasts from dexamethasone-induced oxidative injury and cytotoxicity
Figure 2. miR-107 inhibition causes CAB39 upregulation and AMPK signaling activation in osteoblasts. Stable OB-6 cells with pre-miRNA-107 anti-sense lentivirus (antagomiR-107-L1/L2, two stable cell lines) or control anti-sense lentivirus (antagomiR-C), as well as the parental OB-6 cells were cultured, expression of mature miRNA-107, CAB39 and AMPK signaling proteins was tested by qPCR (A and C) and Western blotting (D and E) assays, with relative CAB39 3’-UTR luciferase activity (B) and AMPK activity (F) examined as well. The primary human osteoblasts were infected with antagomiR-107 lentivirus or antagomiR-C lentivirus for 48h, expression of listed genes was shown (G–J). Data were mean ± standard deviation (SD, n=5). * p<0.05 vs. “antagomiR-C” cells. Each experiment was repeated three times and similar results were obtained.