Research Paper Volume 12, Issue 12 pp 11685—11697

Figure 5. LncRNA KCNQ1OT1 promotes aerobic glycolysis and CRC cell proliferation via HK2. (A) Representative western blot images show HK2 protein levels in parental HCT116 cells (control) and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. (B, C) ECAR assay results show extracellular acidification rate (ECAR) in parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. Each data point represents means ± SD. The experiment was repeated three times independently. (D, E) Lactate assay results show lactate levels in parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. (F, G) Glucose secretion assay results show the concentration of glucose in the media of parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. (H, I) CCK-8 assay results show proliferation status of parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. Note: **p < 0.01, ***p < 0.001, NS, not significant.