Research Paper Volume 12, Issue 12 pp 11636—11652
PLK1 Inhibition alleviates transplant-associated obliterative bronchiolitis by suppressing myofibroblast differentiation
Figure 7. PLK1 siRNA suppresses differentiation of myofibroblasts in vitro and in vivo. (A) Protein expression of PLK1 following siRNA transfection. (B) The mRNA expression of α-SMA following PLK1 siRNA transfection. (C, D) The expression of α-SMA was measured by FCM; quantification by MFI analysis is shown in the bar diagram. (E) The expression of α-SMA was analyzed by IF. (F, G) The expression of α-SMA was analyzed by WB. (H, I) Representative immunoblots for MEK, phosphorylated ERK, and GAPDH at various timepoints are shown. (J) Representative immunohistochemical stains of PLK1 siRNA-treated or control tracheal grafts 28 days post-transplantation. (K) Percentage of luminal occlusion in the PLK1 siRNA-treated and control groups 28 days after transplantation.