Research Paper Volume 12, Issue 12 pp 11550—11567

Figure 5. SENP3-EIF4A1 interacts with miR-9-5p in a direct manner. (A) Cytoplasmic and nuclear levels of SENP3-EIF4A1 in HuH7 and Hep3b cells analyzed by qRT-PCR. (B) The RNA-FISH results verified that SENP3-EIF4A1 was distributed mostly in the cytoplasm in HuH7 cells. (C) MiR-9-5p expression in HCC tissues and adjacent normal tissues detected by qRT-PCR. (D) Bioinformatics evidence of binding of miR-9-5p onto 3'-UTR of SENP3-EIF4A1. (E) RIP experiments for the amount of SENP3-EIF4A1 and miR-9-5p in HuH7 and Hep3b cells. (F) Dual-luciferase reporter gene assay in HuH7 and Hep3b cells after transfection with negative control or miR-9-5p mimics, Renilla luciferase vector pRL-SV40 and the reporter constructs. Data are presented as mean ± SD. *P<0.05. All of the experiments were performed in triplicate.