Research Paper Volume 12, Issue 12 pp 11550—11567

Figure 4. Effect of exosomal SENP3-EIF4A1 on HCC cellular phenotype. Exosomes separated from HL-7702 cells transfected with SENP3-EIF4A1 overexpression plasmids or NC vectors are named SENP3-EIF4A1-Exos or NC-Exos, respectively. After extraction, their exosomes are added to the HuH7 and Hep3b cells for 24h. (A) Detection of the mRNA level of SENP3-EIF4A1 via qRT-PCR. (B) Detection of cell viability by a CCK8 assay. (C) Examination of cell colony formation ability by a colony-forming growth assay. The colonies are counted and captured. (D) Representative images of migration assays of HuH7 and Hep3b cells (bars =100 μm). The cells are counted. Results are shown as mean ± SD. *P<0.05. All of the experiments were performed in triplicate.