miR-137 prevents inflammatory response, oxidative stress, neuronal injury and cognitive impairment via blockade of <i>Src</i>-mediated MAPK signaling pathway in ischemic stroke

Runhui Tian; Bo Wu; Cong Fu; Kaimin Guo

doi:doi:10.18632/aging.103301

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Research Paper Volume 12, Issue 11 pp 10873—10895

miR-137 prevents inflammatory response, oxidative stress, neuronal injury and cognitive impairment via blockade of Src-mediated MAPK signaling pathway in ischemic stroke

Figure 7. Src knockdown reduces cell apoptosis in brain tissues of MCAO mice by down-regulating the MAPK signaling pathway. (A) Immunohistochemistry of ERK1/2 (× 400), p38 (× 400) and Src (× 200) in the brain tissues of Src^-/- mice; (B) diagram based on the immunofluorescence of cleaved caspase 3, TUNEL staining, and DAPI in brain tissues of Src^-/- mice (× 400); (C) mRNA expression of Erk2, Jnk, p38, Gfap, Bax and Bcl-2 in brain tissues of Src^-/- mice using RT-qPCR; (D) protein level of GFAP, Bax and Bcl-2 along with the extent of ERK1/2, JNK and p38 phosphorylation in brain tissues of Src^-/- mice using Western blot analysis. Measurement data are expressed as mean ± standard deviation and compared using one-way ANOVA, followed by Tukey's post hoc test. * p < 0.05 vs. control group (sham-operated wild-type mice); # p < 0.05 vs. MCAO group (wild-type mouse models of MCAO). N = 15.