Figure 7.F2R promotes the malignant behavior of glioma via Wnt signal pathway. (A) GSEA enrichment plots demonstrated that enrichment of MYC targets and WNT signal pathways was associated with up-regulation of F2R. (B and C) Western blot analysis of F2R, c-MYC and β-catenin expression in F2R silenced or overexpressed U87 cells. (D and E) mRNA expression of the β-catenin signal pathway downstream genes (AXIN2, SOX9, CD44 and CCND2) in F2R knockdown or overexpressed U87 cells, which were determined by qPCR. (F and G) Western blot analysis of F2R, c-MYC and β-catenin expression in the U87 and U251 cell lines transfected with SOX2 or/and si-F2R. All of the experiments were performed at least three times. Data are means ± SEM. *P<0.05, **P<0.01.
