This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 11 pp 10544—10555
Long noncoding RNA LINC00460 conduces to tumor growth and metastasis of hepatocellular carcinoma through miR-342-3p-dependent AGR2 up-regulation
Figure 3. LINC00460 interacts with miR-342-3p in HCC cells. (A) Bioinformatics prediction showed the complementary sequence between LINC00460 and miR-342-3p. (B) Relative expression of miR-342-3p in HuH7 cells was detected through qRT-PCR. (C) Luciferase reporter assay was conducted to verify the targeting relationship between LINC00460 and miR-342-3p. (D) Relative expression of miR-342-3p in HuH7 cells was detected through qRT-PCR. (E, F) Western blot showed the expression of CyclinD1, CDK4, MMP-3 and MMP-9 in SNU423 cells. Data statistics was also shown. (G, H) Western blot showed the expression of Bax and Bcl-2 in SNU423 cells. Data statistics was also shown. *P < 0.05, **P < 0.01vs the control group. #P < 0.05, ##P < 0.01 vs the si- LINC00460 group.