Research Paper Volume 12, Issue 11 pp 10441—10456

Figure 6. Overexpression of miR-34-5p ameliorated cardiac fibrosis in the mice after MI. (A) Intravenous injection of AAV9-miR-34-5p via tail increased miR-34-5p expression in normal mice, as measured by qRT-PCR; GAPDH served as an internal control, and AAV9-scramble served as a negative control. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 independent cell cultures. (B, C) Four weeks after MI, echocardiographic imaging showed that the overexpression of miR-34-5p improved ejection fraction (EF) and fraction shortening (FS). Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=12 mice per group. (D) qRT-PCR analysis showing that AAV9-miR-34-5p injection reversed the up-regulation of collagen 1α1 and collagen 3α1 in MI mice; GAPDH mRNA served as an internal control, and AAV9-scramble served as an additional control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. (E) Protein levels of collagen 1 and α-SMA were measured by western blot; GAPDH served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. **P<0.05.