Research Paper Volume 12, Issue 11 pp 10398—10414

Figure 3. Effects of TP supplementation on complex V subunit expression in the substantia nigra of aged male rats. (A–C) The mRNA levels of ATP6, ATP8 and ATP5C1 were calculated using the 2^-ΔΔCt method. GAPDH was used as an internal control. (D–F) Representative Western blots of ATP6, ATP8 and ATP5C1 protein levels. (G–I) ATP6, ATP8 and ATP5C1 protein levels were quantified by comparing the band density of each protein to that of β-actin (endogenous control). Data are expressed as the mean ± S.D. (n=6 rats/group). ^*P<0.05, ^**P<0.01.