Research Paper Volume 12, Issue 12 pp 11466—11484

Figure 2. Modulation of AR regulate the immune state. (A) Schematic diagram of PD1-PD-L1 binding assay. (B) The results of binding assay in three HCC cell lines. (C) Intracellular INF-r expression in CD8⁺T cells co-cultured with HCC cells. (D) Intracellular TNF-a expression in CD8⁺T cells co-cultured with HCC cells. (E) Secreted cytokine (granzyme B and perforin) in MHCC97H cells. (F) ELISA of serum secreted cytokine (granzyme B and perforin) in HCCLM3 cells. (G) T cell cytotoxicity assay in MHCC97H with different AR expression. (H) AR antagonist cause change of AR and PD-L1 in vitro. *P<0.05, ** P<0.01 and *** P<0.001.