Research Paper Volume 12, Issue 8 pp 7576—7584

Figure 1. Influence of 1nM Rapamycin supplementation on early embryonic development. The results are presented as cleavage rates and blastocyst rates in % (total number of metaphase II oocyte/total number of embryonic stage). (A) shows the results for proper cleaved embryos on day 4 in the three experimental groups. (B) shows the results for the blastocyst rate on day 7 in the three experimental groups. (C) shows the results for the blastocyst rate on day 8 in the three experimental groups. (D) shows the results for the blastocyst rate on day 9 in the three experimental groups. A total of 695 good quality COCs (representative COCs shown in (E) [scale bar: 100 μm] and (F) [scale bar: 50 μm]) were used for the in vitro maturation, fertilization, and culture until day 9, and the "n" above each bar represents the number of detected good quality early cleavage stage embryos, (control= control without supplementation, CDM=vehicle control with DMSO supplementation, 1nMRap= 1nM Rapamycin supplementation).