PGC-1α activator ZLN005 promotes maturation of cardiomyocytes derived from human embryonic stem cells

Yanping Liu; Huajun Bai; Fengfeng Guo; Phung N. Thai; Xiaoling Luo; Peng Zhang; Chunli Yang; Xueqin Feng; Dan Zhu; Jun Guo; Ping Liang; Zhice Xu; Huangtian Yang; Xiyuan Lu

doi:doi:10.18632/aging.103088

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Research Paper Volume 12, Issue 8 pp 7411—7430

PGC-1α activator ZLN005 promotes maturation of cardiomyocytes derived from human embryonic stem cells

Figure 6. ZLN005-treated hESC-CMs displayed an increase in calcium signaling and kinetics compared with control. (A) qRT-PCR analysis of cardiac calcium handling markers in control and ZLN005-treated hESC-CMs (n=6). (B) Representative intracellular Ca²⁺ transients from control (red trace) and ZLN005-treated (black trace) hESC-CMs. Calcium transients were evaluated by loading the hESC-CMs with fura-2 AM. (C–G) Ca²⁺ transients properties of control and ZLN005-treated ESC-CMs: The amplitude of Ca²⁺ transient (C) time to peak (D) maximal velocity of upstroke (E) decay time (F) maximal velocity of decay (G). n=15-20 cells for each group.