Research Paper Volume 12, Issue 8 pp 7232—7247

Figure 3. MiR-101a-3p participates in the regulation of Fos expression. (A) Bioinformatic analysis (http://www.microrna.org/) showed that FOS was a target of miR-101a-3p (upper panel) and the luciferase activity was analysed (lower panel) (n=3). FOS-WT: wild-type FOS, FOS-Mut: FOS with mutated 3’-UTR. (B) The results of RNA pull-down assay (n=3). Nc mimic: a sequence did not interact with FOS; Con: control. (C) The expression of FOS was measured by qRT-PCR in NMCMs under normal or anoxia condition. The application of miR-101a-3p mimic down-regulated FOS expression (n=3). (D) The protein level of c-Fos was measured by western blot in in NMCMs under normal or anoxia condition. The application of miR-101a-3p mimic down-regulated C-FOS expression (n=3). Data are shown as mean ± SD, * P<0.05.