Research Paper Volume 12, Issue 8 pp 6904—6927

Figure 4. CDK6 knockdown inhibited gastric cancer development in vitro. (A, B) Cell models with or without CDK6 knockdown were constructed by transfecting shCDK6 or shCtrl. The knockdown efficiency of UAP1L1 in SGC-7901 cells was assessed by qPCR (A) and western blotting (B). (C) Celigo cell counting assay was employed to show the effects of CDK6 on cell proliferation of SGC-7901 cells. (D) Colony formation assay was used to evaluate the ability of SGC-7901 cells with or without CDK6 knockdown to form colonies. (E) Flow cytometry was performed to detect cell apoptosis of SGC-7901 cells with or without CDK6 knockdown. (F, G) The effects of CDK6 on cell migration ability of SGC-7901 cells were evaluated by wound-healing assay (F) and Transwell assay (G). The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.