Research Paper Volume 12, Issue 8 pp 6865—6879

Figure 2. circRGNEF expression was also increased in BC cell lines and knockdown of circRGNEF suppressed cell proliferation. (A) RT-qPCR detection of circRGNEF expression in BC cell lines EJ, T24, J82, UM-UC-3, TCC, and RT-4, and the normal cell line SV-HUC. Data are presented as the mean ± SD. ^***P < 0.001 vs. SV-HUC. (B) RT-qPCR detection shows the expression of circRGNEF in both T24 and UM-UC-3 cells following transfection with small interfering RNA targeting circRGNEF (si-circRGNEF) or negative control (NC). Data are presented as the mean ± SD. ^***P < 0.001 vs. NC. (C) Cell cycle distribution by flow cytometry after propidium iodide staining. (D, E) CCK8 assay shows the proliferation of T24 and UM-UC-3 cells with or without circRGNEF silencing. Data are presented as the mean ± SD. ^***P < 0.001 vs. NC. (F) Colony formation assay was performed to determine the colony-forming ability of T24 and UM-UC-3 cells. (G, H) circNRIP1 silencing significantly inhibited DNA synthesis, as determined by the EdU assay. Data are presented as the mean ± SD. ^***P < 0.001 vs. NC.