Research Paper Volume 12, Issue 8 pp 6823—6851

Figure 1. Psoriatic keratinocyte cultures display enhanced IGFBP2 expression, together with an altered expression of genes implicated in the regulation and cell cycle arrest. (A) Real-time PCR analysis was performed on keratinocyte cultures (at passage P4), obtained from lesional skin of psoriatic patients (n = 6) (pso KC) and healthy volunteers (n = 6) (healthy KC). Results are shown as individual values of relative mRNA levels (normalized to β-actin) of IGFBP2, IGFBP3, p16, p21 Cdk1, cyclin A and p57 and means of the two different groups. (B) WB analysis was performed on protein lysates from keratinocyte cultures isolated from healthy (n = 6) and lesional skin (n = 6) by using anti-IGFBP2, cyclin A, cdk1, -p16 and -p21 Abs. β-actin was used as loading control. Bands relative to IGFBP2 were showed at two different exposure times (High exp. 1 min; low exp., 30 seconds). Graphs represent the individual values and the means of the densitometric intensity (D.I.) of each band. (A, B), *p ≤ 0.05, as calculated by the Mann–Whitney U test.