Research Paper Volume 12, Issue 8 pp 6644—6666

Figure 8. The Sox2OT-V7/miR-142/miR-22 axis modulates autophagy in OS cells by regulating autophagy-related genes. (A, B) U2OS cells were cotransfected with Lsh-Sox2OT-V7 and miR-142 inhibitor under Dox treatment and the protein levels of ULK1, ATG4A, and ATG5 were examined. (C, D) U2OS cells were cotransfected with Lsh-Sox2OT-V7 and miR-22 inhibitor under Dox treatment the protein levels of ULK1. (E–H) U2OS cells were transfected with si-ULK1, si-ATG4A, or si-ATG5 under Dox treatment and the protein levels of LC3 II, Beclin 1, and p62 were examined using immunoblotting. The data are presented as the mean ± SD of three independent experiments. *P<0.05, **P<0.01, compared to the control group; ##P<0.01, compared to the miR-142 inhibitor or miR-22 inhibitor group. (I) U2OS cells with stable eGFP-LC3 expression were transfected with si-ULK1, si-ATG4A, or si-ATG5 under Dox treatment, and the formation of puncta was examined by using a confocal microscope. Representative images are presented.