Research Paper Volume 12, Issue 4 pp 3862—3879

Figure 5. The anti-inflammatory effects of acetate in BV2 cells (n=3 independent experiments). During the acetate post-treatment, different concentrations of acetate (10, 20 or 50 mM) were added to the wells 30 min after LPS (100 ng/mL) stimulation. Inflammatory proteins were detected by ELISA 6 h after LPS stimulation. Bar graphs display the protein levels of TNF-α (A) and IL-6 (B). During the acetate pretreatment, acetate was added to the wells 30 min before LPS (100 ng/mL) stimulation, and inflammatory proteins were detected by ELISA as previously mentioned. Bar graphs display the protein levels of TNF-α (C) and IL-6 (D). (E) The MTT experiment revealed no significant differences in cell viability among the groups. Data are expressed as the mean±SEM, *P<0.05 vs. the control and acetate groups, #P<0.05 vs. the LPS group. ACE: acetate.