Figure 4. Co-culturing-induced changes in visibility of PGAM2 C-terminus, immunostaining intensity of TIGAR, and ROS and lactate production. PGAM2 is presented in white, nuclei are blue. White arrow point to cardiomyocytes. Bar = 10 μm. Lactate production is presented as pmol of lactate per cell per 48 h. Error bars represent standard deviation from three measurements. RFU – relative fluorescence units.