Research Paper Volume 12, Issue 4 pp 3266—3286
Orai, STIM, and PMCA contribute to reduced calcium signal generation in CD8+ T cells of elderly mice
Figure 5. mRNA and protein levels of distinct STIM and Orai isoforms are reduced in CD8+ T cells of elderly mice. (A) Normalised relative mRNA expressions of Orai1, 2 and 3 and STIM1 and 2 of untouched CD8+ T cells from adult (grey, n = 12) and elderly (red, n = 11) mice. (C) Normalised relative mRNA expressions of SOCE components of stimulated CD8+ T cells from adult (grey, n = 12) and elderly (red, n = 11) mice. Protein quantification after normalization to GAPDH of SOCE components from (B) untouched (n = 7 - 9) and (D) stimulated (n = 5 - 7) CD8+ T cells lysates isolated from adult (black) and elderly (red) mice. Data obtained are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.