Figure 4.Metastatic gastric cancer cell supernatants significantly altered the morphology of peritoneal mesothelial cells. HMrSV5 cells were co-cultured with NUGC-4, NUGC-4per, NUGC-4liver, NUGC-4lym cell supernatants and NUGC-4per cell supernatants + TGF-β inhibitor for 48, 72 and 96 h. (A) The morphology of HMrSV5 cells was observed under an optical microscope at 48, 72 and 96 h. (B) DAPI and F-actin were detected by an immunofluorescence assay in HMrSV5 cells co-cultured with NUGC-4, NUGC-4per, NUGC-4liver, NUGC-4lym cell supernatants and NUGC-4per cell supernatants + TGF-β inhibitor for 72 h. (C–E) The relative levels of p-Smad2 and p-Smad3 in peritoneal mesothelial cells were detected by western blotting. **P<0.01 compared to the control group.
