Research Paper Volume 11, Issue 22 pp 10532—10556

Figure 9. LH improves the DNA damage repair capability by somatic ovarian cells. (A) Representative IF of cells stained with MLH1 (green) after 16, 24, 36, 48 and 72 hrs of treatment with 10 μM CS with/out 200 mIU/mL LH. Scale bar = 100 μm. (B) The graph reports the Mean Correlated Total Cell Fluorescence (CTCF) in ovarian cells treated with/out CS and CS+LH as indicated. The fluorescence intensity was determined in each cell by ImageJ software. Data are expressed as means ± SEM of three experiments. Statistical differences vs control ***p<0.001 ****p<0.0001; statistical difference of CS group vs CS+LH group *p<0.05 ****p<0.0001; CS 16 hrs vs CS 24 hrs a p<0.05; CS 36 hrs vs CS 48 hrs d p<0.0001; CS 48 hrs vs CS 72 hrs d p<0.0001; LH 16 hrs vs CS+LH 16 hrs d p<0.0001; CS 16 hrs vs CS 24 hrs d p<0.0001; CS 24 hrs vs CS 36 hrs d p<0.0001; CS 36 hrs vs CS 48 hrs d p<0.0001. Note that cells showed a progressive increase in the nuclear expression of MLH1 already after 16 hrs of treatment with Cs+LH.