Research Paper Volume 11, Issue 21 pp 9738—9766
Selected by gene co-expression network and molecular docking analyses, ENMD-2076 is highly effective in glioblastoma-bearing rats
Figure 7. (A) Transmission Electron Microscope (TEM) images showed that ENMD-2076 induces apoptosis bodies and pyknosis formation in U87 cells. (B–C) Flow cytometry showed that ENMD-2076 induced cell cycle arrest in G2-M phase for LN18, T98G cells. (D) Western blot analysis showed that ENMD-2076 increased the expression of Caspase-3 and Bax in LN18, T98G cells and decreased the expression of anti-apoptotic Bcl-2. Western blot analysis also showed the effects of ENMD-2076 on the phosphorylation levels of Cdc2, cyclin B1 and cyclin D1 in LN18 and T98G cell lines. (E) Western blot analysis showed that ENMD-2076 suppressing PI3K/Akt/mTOR signaling pathway in LN18, T98G cells.