Research Paper Volume 11, Issue 21 pp 9689—9708

Figure 6. circGRAMD1B alleviates GC cell proliferation, migration, and invasion by targeting miR-130a-3p. (A) miR-130a-3p overexpression or silencing was performed in MGC803 and AGS cells with miR-130a-3p mimics or inhibitors, respectively. (B–C) CCK-8 assays were used to assess the proliferation abilities of the transfected MGC803 and AGS cells. MGC803 cells were transfected with the miR-NC, the circGRAMD1B-overexpressing plasmid, or the circGRAMD1B-overexpressing plasmid and miR-130a-3p mimics; AGS cells were transfected with the miR-NC, circGRAMD1B siRNA1^# or circGRAMD1B siRNA1^# and miR-130a-3p inhibitors. (D) Colony formation assays were used to evaluate the proliferation abilities of the transfected MGC803 and AGS cells. (E) Western blot assays were used to analyze the protein expression levels of PCNA and Ki-67 in transfected MGC803 and AGS cells. (F–G) Transwell assays were performed to evaluate the migration and invasion abilities of transfected MGC803 and AGS cells. (H) Western blot assays were used to analyze the protein expression levels of E-cadherin and Vimentin in transfected MGC803 and AGS cells. (Values are shown as the mean ± standard error of the mean based on three independent experiments. *P < 0.05, **P < 0.01).